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KMID : 0368420100530040251
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Journal of Plant Biology
2010 Volume.53 No. 4 p.251 ~ p.258
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Isolation and Characterization of a Putative Vacuolar Na+/H+ Antiporter Gene from Zoysia japonica L.
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Du Yanhua
Hei Qian
Liu Yaxin
Zhang Hui
Xu Kai
Xia Tao
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Abstract
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A Na+/H+ antiporter gene from Zoysia japonica (ZjNHX1) which is a member of plant NHX-genes family was cloned by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology. The isolated cDNA is 2,421 bp in length and contains one open-reading frame (ORF) which comprises 540 amino acid residues and shows higher homology with other plant Na+/H+ antiporters. ZjNHX1 could partially complement the salt-sensitive phenotypes of ?nhx1 and ?ena1-4?nhx1 yeast mutants in the presence of NaCl, KCl, and LiCl. The expression of ZjNHX1 in Z. japonica increased after NaCl treatment and this result accords with that of Na+ contents determination under the same treatment. These results implied that ZjNHX1 functions as a vacuolar Na+/H+ antiporter and plays an important role in salt tolerance and ion homeostasis in Z. japonica.
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KEYWORD
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Ion homeostasis, Na+/H+ antiporter, Salt tolerance, Zoysia japonica
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